ABSTRACT
The purpose of the present work is to observe whether Tau protein Ser202/Thr205 is hyperphosphorylated in braintissues of diabetic mice and to study the effect of App17 peptide. Mouse diabetic model was produced with streptozotocin, andApp1 7 peptide as a treatment was injected subcutaneously into diabetic mice. Four weeks later, fixative was injected intravascu-larly into the mice, the brain was removed and crystat sections prepared. Immunohistochemical staining was done with AT-8. Inthe brains of diabetic mice positive AT-8 reacting neurons were numerous, darkly stained, and widely distributed in retrosplenialgranular cortex, hippocampus, thalamus et al. , while in normal mice and App17 peptide-treated diabetic mice positive cells werescarce and poorly stained. Tau protein is hyperphosphorylated at Scr202/Thr205 site and widely distributed in the brains of dia-betic mice, while App17 peptide can normalize the expression of AT-8 positive cells.
ABSTRACT
Horseradish peroxidase (HRP) was injected into the stellate ganglia of anesthetized cats. After 52-72 hour survival time, the animals were perfused and the spinal cord was processed by O-D technique to study the localization and segmental distribution of the sympathetic preganglionic neurons in the spinal cord which project to the stellate ganglia. HRP-labeled neurons in the spinal cord were located exclusively on the side of the injection and were found in five distinct areas:1. nucleus intermediolateralis pars principalis (ILp 76.08%).2. nucleus intermediolateralis pars funicularis (ILf 10.51%).3. anterior horn (AH 5.88%).4. nucleus intercalatus (IC 5.31%).5. nucleus intercalatus pars paraependymalis (ICpe 2.22%).Labeled cells were found in C_8-T_(10) spinal cord segments, with the highest accumulation of cells in T_3 segment. Furthermore, HRP-labeled ILp neurons were detected in C_8-T_(10) spinal cord segments, with a peak at T_3 segment; ILf neurons were seen in 8-T_9, with a peak at T_1 segment; AH neurons were seen in T_1-T_8, with a peak at T_2 segment; IC and ICpe neurons were seen in C_8-T_(10), with a peak at T_6 segment. Stellate ganglion; Sympathetic preganglionic neuron; Spinal cord; HRP method; Cat
ABSTRACT
The segmental distribution of the afferent fibers traversing the stellate ganglia was studied with HRP techinque in 33 cats. The animals were divided into two groups. In one group HRP was injected into the stellate ganglion of one side. Labelled cells were found in ipsilateral spinal ganglia from C_4 to T_9. The majority of these cells (84.49％) were distribued in T_1 to T_5 segments. There is no conspicuous difference between the left and right in respect to the segmental and the pattern distribution of labelled cells. The majority of the labelled cells were medium or small sized (